Pauline Hanson ruthlessly mocks Robert Irwin after the wildlife warrior threatened to take her to court over satirical cartoon

Despite being threatened with legal action, Pauline Hanson has once again targeted Robert Irwin in a new episode of her satirical animated series.

Irwin is not the only target in the latest episode of Ms Hanson’s controversial Please Explore series, titled ‘The State of Melbourne’.

Bluey, Anthony Albanese, former Victoria Premier Dan Andrews and former CFMEU leader John Setka are also in the One Nation leader’s crosshairs.

The latest release comes just months after Irwin threatened the producers with legal action over an earlier episode that attacked the Queensland Government and Irwin for their role in a Queensland tourism campaign.

The latest animated episode was uploaded to Ms Hanson’s YouTube channel on Friday.

It starts with ‘Irwin’ addressing the viewer: ‘Yes, g’day, it’s me again Robert Irwin…

“You know, when I did that ad in Queensland, a lot of people said I was just picking my family name for financial gain.

“Well, you haven’t seen anything yet because now I’m doing a shameless sequel for Tourism Melbourne with my best friend Bluey.”

In the video, the characters Irwin and Bluey are run over by the caricatures of Mr Andrews and Mr Albanese, who then continue to film the tourism advert.

Pauline Hanson once took aim at Robert Irwin in the latest episode of her controversial Please Explain series, titled ‘The State of Melbourne’

'Robert Irwin' and 'Bluey' feature in the latest episode in a parody of an advert for Melbourne Tourism

‘Robert Irwin’ and ‘Bluey’ feature in the latest episode in a parody of an advert for Melbourne Tourism

Mr. Andrews plays Irwin and Mr. Albanese is Bluey.

The episode also focuses on a crack in a safe injection room in the Melbourne suburb of Richmond and the city’s sustainable transport system.

Elsewhere, a caricature of former CFMEU leader John Setka is seen pushing a wheelbarrow full of cash.

The video has already racked up almost 10,000 likes, with Ms Hanson’s followers praising the video.

‘I live in Melbourne and I can confirm this video is 100% accurate!’ wrote one.

‘Elected 1 One Nation yesterday. We need Pauline as Prime Minister. Then I didn’t wake up,” another wrote.

“Our favorite Friday morning watch before school with my son…… LOVE THIS!!,” wrote a third.

In the video, characters Dan Andrews and Anthony Albanese run across 'Robert Irwin' and 'Bluey'

In the video, characters Dan Andrews and Anthony Albanese run across ‘Robert Irwin’ and ‘Bluey’

Ms Hanson’s ‘right-hand man’ James Ashby, who is credited as the video’s producer, said One Nation had the right to publish the satirical series despite Irwin’s legal threat.

“It’s just a bit of fun,” he told the Courier Mail.

‘We’ve been advised by some of the best defamation specialists in the country who tell us it’s not defamatory, so it’s certainly a tongue-in-cheek comment, but everyone should at this time of year, especially with the election approaching, good laugh.

“Instead of paying lawyers, Robert can always call us.”

The Please Explain series is no stranger to controversy, with one episode limited to social media during the 2022 federal election.

Former CFMEU leader John Setka is also in the One Nation leader's crosshairs in her latest video

Former CFMEU leader John Setka is also in the One Nation leader’s crosshairs in her latest video

The cartoon showed a character based on Labor senator Penny Wong handing out a series of fake votes to her bedridden, Covid-stricken leader, Anthony Albanese.

The skit led to a warning from the Australian Electoral Commission and later removal or restriction by several social media platforms.

The AEC said Ms Hanson’s attempts to undermine democratic trust were ‘extremely disappointing’.

“Aspects of it are clearly false, and any registered party would know this,” the AEC said.

“No election that the AEC has organized has had a margin smaller than the number of alleged multiple votes [and] Proof of identification is required when registering to vote.”